Multiphoton microscope

LaVision BioTec TriM Scope II

Powered by an Insight X3 laser (tunable 680 - 1300 nm + fixed 1040 nm outputs), this microscope comprises dual scan lines for synchronous deep tissue imaging and photomanipulation. The automated stage enables multi-position imaging and volumetric tile scans.  Adaptive optics allows for aberration correction. A 3-axis closed-loop piezo objective stage reduces movement artefact during in vivo imaging.  Custom upgrades enable two-photon STED and confocal imaging 

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Widefield/Superresolution microscope (Zeiss Axio Imager.A2)

This phase contrast fluorescence microscope also operates as a real-time super-resolution microscope using Super-Resolution Radial Fluctuations (SRRF-Stream)

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Surgical microscope (Leica Wild M651)

Provides high resolution magnification for microsurgery on live rodent models.

Graphics Workstation 1: Imaris, Fiji

 

AVAdirect (i9-9980XE 3.0 GHz 18 cores, 128GB RAM, AMD Radeon RX 580 DUAL x1 Graphics Card, Microsoft Windows 10 Professional Edition 64bit OS)

High-end PC for graphics work running Imaris and Fiji (ImageJ). System can be used for quantitation and analysis of data. This System is primarily used for image processing/analysis with Imaris and GPU accelerated multiview deconvolution of Lightsheet data using Fiji. This system is primarily used for conversion of imspector files to .tif format and GPU accelerated image processing/analysis for 3D rendering, stitching and super-resolution radial fluctuations (SRRF-Stream).

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Graphics Workstation 2: Aivia, Matlab

AVAdirect(i9-7900X 3.3 GHz 10 cores, 96 GB RAM, NVIDIA Quadro RTX 5000 x 1  Graphics Card, Microsoft Windows 10 Professional Edition 64bit OS )

High-end PC for graphics work running Aivia and Matlab R2020a. System can be used for image restoration, 3D segmentation, and objects detection. This system is primarily used for GPU accelerated 3D neuron analysis with a machine learning software (AIVIA) for image deconvolution and axon quantification.

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Graphics Workstation 3: Python, DeepLabCut

PUGET (Dual XEON processors E5-2687W 3.10 GHz 16 cores, 256 GB RAM, NVIDIA Quadro M6000 x1 Graphics Card, Microsoft Windows 10 Professional Edition 64bit OS)

 

High-end PC for graphics work running and Python 3.8.3 and DeepLabCut. System can be used to load images in napari for image analysis based on custom Python functions. This system is primarily used for DeepLabCut, a GPU accelerated toolbox for markerless pose estimation of rats (automated whisker tracking and eyes blinking), and landmarks of the human body (face, vocal fold, etc).

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